{
 "cells": [
  {
   "cell_type": "code",
   "execution_count": 1,
   "metadata": {
    "collapsed": true
   },
   "outputs": [],
   "source": [
    "using Gadfly\n",
    "using ColorBrewer\n",
    "using DataFrames"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {
    "collapsed": false
   },
   "outputs": [],
   "source": [
    "df = readtable(\"../data/bigiron_scan_rep_space_a496065_clean.csv\");"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "metadata": {
    "collapsed": false
   },
   "outputs": [
    {
     "data": {
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       "<table class=\"data-frame\"><tr><th></th><th>method</th><th>measure</th><th>genetype</th><th>screen</th><th>transfection_representation</th><th>bottleneck_representation</th><th>sequencing_representation</th><th>crisprtype</th><th>std_score</th><th>mean_score</th><th>score_max</th><th>score_min</th><th>n</th></tr><tr><th>1</th><td>auprc</td><td>dec</td><td>all</td><td>FacsScreen</td><td>1.0</td><td>1.0</td><td>1.0</td><td>CRISPRKO</td><td>0.04608391290455255</td><td>0.18599032665579845</td><td>0.21455343179695596</td><td>0.15742722151464095</td><td>10</td></tr><tr><th>2</th><td>auprc</td><td>dec</td><td>all</td><td>FacsScreen</td><td>1.0</td><td>1.0</td><td>1.0</td><td>CRISPRi</td><td>0.04207112470955607</td><td>0.20149441434864723</td><td>0.2275703675988559</td><td>0.17541846109843856</td><td>10</td></tr><tr><th>3</th><td>auprc</td><td>dec</td><td>all</td><td>FacsScreen</td><td>1.0</td><td>1.0</td><td>2.154434690031884</td><td>CRISPRKO</td><td>0.04277382857145645</td><td>0.17882652444772412</td><td>0.2053380180638763</td><td>0.15231503083157194</td><td>10</td></tr><tr><th>4</th><td>auprc</td><td>dec</td><td>all</td><td>FacsScreen</td><td>1.0</td><td>1.0</td><td>2.154434690031884</td><td>CRISPRi</td><td>0.05941812919789614</td><td>0.28650094931424874</td><td>0.3233286873382638</td><td>0.24967321129023368</td><td>10</td></tr><tr><th>5</th><td>auprc</td><td>dec</td><td>all</td><td>FacsScreen</td><td>1.0</td><td>1.0</td><td>4.641588833612778</td><td>CRISPRKO</td><td>0.04088895860236218</td><td>0.19290118847436327</td><td>0.21824442758017998</td><td>0.16755794936854657</td><td>10</td></tr><tr><th>6</th><td>auprc</td><td>dec</td><td>all</td><td>FacsScreen</td><td>1.0</td><td>1.0</td><td>4.641588833612778</td><td>CRISPRi</td><td>0.06897681157999591</td><td>0.27778119196349393</td><td>0.3205334627079906</td><td>0.23502892121899727</td><td>10</td></tr></table>"
      ],
      "text/plain": [
       "6×13 DataFrames.DataFrame\n",
       "│ Row │ method  │ measure │ genetype │ screen       │\n",
       "├─────┼─────────┼─────────┼──────────┼──────────────┤\n",
       "│ 1   │ \"auprc\" │ \"dec\"   │ \"all\"    │ \"FacsScreen\" │\n",
       "│ 2   │ \"auprc\" │ \"dec\"   │ \"all\"    │ \"FacsScreen\" │\n",
       "│ 3   │ \"auprc\" │ \"dec\"   │ \"all\"    │ \"FacsScreen\" │\n",
       "│ 4   │ \"auprc\" │ \"dec\"   │ \"all\"    │ \"FacsScreen\" │\n",
       "│ 5   │ \"auprc\" │ \"dec\"   │ \"all\"    │ \"FacsScreen\" │\n",
       "│ 6   │ \"auprc\" │ \"dec\"   │ \"all\"    │ \"FacsScreen\" │\n",
       "\n",
       "│ Row │ transfection_representation │ bottleneck_representation │\n",
       "├─────┼─────────────────────────────┼───────────────────────────┤\n",
       "│ 1   │ 1.0                         │ 1.0                       │\n",
       "│ 2   │ 1.0                         │ 1.0                       │\n",
       "│ 3   │ 1.0                         │ 1.0                       │\n",
       "│ 4   │ 1.0                         │ 1.0                       │\n",
       "│ 5   │ 1.0                         │ 1.0                       │\n",
       "│ 6   │ 1.0                         │ 1.0                       │\n",
       "\n",
       "│ Row │ sequencing_representation │ crisprtype │ std_score │ mean_score │\n",
       "├─────┼───────────────────────────┼────────────┼───────────┼────────────┤\n",
       "│ 1   │ 1.0                       │ \"CRISPRKO\" │ 0.0460839 │ 0.18599    │\n",
       "│ 2   │ 1.0                       │ \"CRISPRi\"  │ 0.0420711 │ 0.201494   │\n",
       "│ 3   │ 2.15443                   │ \"CRISPRKO\" │ 0.0427738 │ 0.178827   │\n",
       "│ 4   │ 2.15443                   │ \"CRISPRi\"  │ 0.0594181 │ 0.286501   │\n",
       "│ 5   │ 4.64159                   │ \"CRISPRKO\" │ 0.040889  │ 0.192901   │\n",
       "│ 6   │ 4.64159                   │ \"CRISPRi\"  │ 0.0689768 │ 0.277781   │\n",
       "\n",
       "│ Row │ score_max │ score_min │ n  │\n",
       "├─────┼───────────┼───────────┼────┤\n",
       "│ 1   │ 0.214553  │ 0.157427  │ 10 │\n",
       "│ 2   │ 0.22757   │ 0.175418  │ 10 │\n",
       "│ 3   │ 0.205338  │ 0.152315  │ 10 │\n",
       "│ 4   │ 0.323329  │ 0.249673  │ 10 │\n",
       "│ 5   │ 0.218244  │ 0.167558  │ 10 │\n",
       "│ 6   │ 0.320533  │ 0.235029  │ 10 │"
      ]
     },
     "execution_count": 3,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "grouped_info = by(df, [:method, :measure, :genetype, :screen, :representation, :bottleneck_representation, :seq_depth, :crisprtype]) do grouped_df\n",
    "    n = size(grouped_df, 1)\n",
    "    mean_score = clamp(mean(grouped_df[:score]), 0, 1)\n",
    "    std_score = std(grouped_df[:score])\n",
    "    conf_int = 1.96 * std_score./sqrt(n)\n",
    "    DataFrame(\n",
    "        std_score = std_score,\n",
    "        mean_score = mean_score,\n",
    "        score_max = mean_score + conf_int,\n",
    "        score_min = mean_score - conf_int,\n",
    "        n = n\n",
    "    )\n",
    "end\n",
    "rename!(grouped_info, Dict(:representation => :transfection_representation,\n",
    ":seq_depth => :sequencing_representation))\n",
    "n_steps = length(unique(grouped_info[:transfection_representation]))\n",
    "rep_vals = hcat(map(x->round(Int64, x), logspace(0,4,n_steps)), logspace(0, 4, n_steps))\n",
    "for repcol in [:transfection_representation, :bottleneck_representation, :sequencing_representation]\n",
    "    arr = zeros(size(grouped_info, 1))\n",
    "    for i in 1:size(rep_vals, 1)\n",
    "        arr[grouped_info[repcol] .== rep_vals[i, 1]] = rep_vals[i, 2]\n",
    "    end\n",
    "    grouped_info[repcol] = arr\n",
    "end\n",
    "head(grouped_info)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "metadata": {
    "collapsed": false
   },
   "outputs": [
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    "selection = [(:genetype, \"all\"), (:method, \"venn\"), (:measure, \"incdec\"), (:crisprtype, \"CRISPRi\")]\n",
    "data = grouped_info[vec(all(hcat([(grouped_info[item[1]] .== item[2]) for item in selection]...), 2)), :];\n",
    "data = vcat([data[data[:sequencing_representation] .== seq_depth, :] for seq_depth in [1.0, 10.0, 100.0, 1000.0, 10000.0]]...)\n",
    "data[!isfinite(data[:mean_score]), :mean_score] = 0.0\n",
    "\n",
    "p = plot(data, ygroup=:sequencing_representation, xgroup=:screen, x=:transfection_representation, \n",
    "y=:bottleneck_representation, color=:mean_score, Geom.subplot_grid(Geom.rectbin,\n",
    "Coord.cartesian(xmin=0, xmax=4, ymin=0, ymax=4)),\n",
    "Scale.x_log10, Scale.y_log10, Scale.color_continuous(),\n",
    "Guide.title(\"$(unique(data[:crisprtype])[1]) using $(unique(data[:method])[1]) scoring\"))\n",
    "draw(SVG(\"../plots/scan_rep_space.svg\", 12cm, 20cm), p)\n",
    "draw(SVG(12cm, 20cm), p)"
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  {
   "cell_type": "code",
   "execution_count": 5,
   "metadata": {
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     "metadata": {},
     "output_type": "display_data"
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   ],
   "source": [
    "selection = Tuple{Symbol, Any}[\n",
    "    (:genetype, \"all\"), \n",
    "    (:method, \"auprc\"), \n",
    "    (:measure, \"incdec\"), \n",
    "]\n",
    "colors = palette(\"Accent\", 4)\n",
    "reps = [:transfection_representation, :bottleneck_representation, :sequencing_representation]\n",
    "datas = []\n",
    "for reptype in combinations(reps, 2)\n",
    "    push!(selection, (reptype[1], 10^4))\n",
    "    push!(selection, (reptype[2], 10^4))\n",
    "    data = grouped_info[vec(all(hcat([(grouped_info[item[1]] .== item[2]) for item in selection]...), 2)), :]\n",
    "    colname = pop!(setdiff(Set(reps), Set(reptype)))\n",
    "    data[:reptype] = colname\n",
    "    data[:representation] = data[colname]\n",
    "    push!(datas, data)\n",
    "    pop!(selection)\n",
    "    pop!(selection)\n",
    "end\n",
    "datas = vcat(datas...)\n",
    "p = plot(datas, xgroup=:screen, ygroup=:crisprtype, x=:representation, \n",
    "y=:mean_score, ymax=:score_max, ymin=:score_min,\n",
    "color=:reptype, Scale.x_log10, Geom.subplot_grid(Geom.line, Geom.ribbon, \n",
    "Guide.yticks(ticks=collect(0:0.2:1)), Coord.cartesian(ymax=1.0, ymin=0.0)),\n",
    "Scale.color_discrete_manual(colors[1:3]..., levels=reps),\n",
    "Theme(line_width=2pt), Guide.title(\"$(unique(datas[:method])[1])\"))\n",
    "draw(SVG(\"../plots/scan_rep_space_line.svg\", 20cm, 20cm), p)\n",
    "draw(SVG(20cm, 20cm), p)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {
    "collapsed": true
   },
   "outputs": [],
   "source": []
  }
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